Next-generation sequencing technologies have allowed new tools to be developed for transcriptome characterization. We describe here the methods for the preparation of a library from pooled RNAs for 454 sequencing of 3'-cDNA fragments. We also describe how the read sequences obtained can be used to generate, through de novo reads assembly, a large catalog of unique transcripts in organisms for which a comprehensive collection of transcripts or the complete genome sequence is not available. Finally, this catalog can be used efficiently to design oligonucleotide probes for setting up a comprehensive microarray for global analysis of gene expression.
CITATION STYLE
Bellin, D., Ferrarini, A., & Delledonne, M. (2011). Generation of a large catalog of unique transcripts for whole-genome expression analysis in nonmodel species. Methods in Molecular Biology (Clifton, N.J.), 729, 257–269. https://doi.org/10.1007/978-1-61779-065-2_17
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