A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5′-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges.
CITATION STYLE
Stults, D. M., Killen, M. W., & Pierce, A. J. (2014). The sister chromatid exchange (SCE) assay. Methods in Molecular Biology, 1105, 439–455. https://doi.org/10.1007/978-1-62703-739-6_32
Mendeley helps you to discover research relevant for your work.