Deleterious effects of nifedipine on smooth muscle cells implies alterations of intracellular calcium signaling

Abstract

Nifedipine (NIF), a calcium channel blocker (CCB) from the first generation of dihydropyridines, induces detrimental effects on patients with cardiovascular diseases. We designed experiments to study, at cellular and molecular level, the mechanisms involved in the induction of deleterious effects by this drug. To this purpose, cultured human smooth muscle cells (HSMC) were used. The effect of NIF and two other CCB (FEL, AML) and inhibitors of intracellular signaling pathways (RR, TG, CAF and GEN) on intracellular calcium [Ca2+]I was determined by spectrofluorimetry using Fura 2 AM assay. The results showed that: (i) 10 μM NIF induced the increase of [Ca2+]I above the basal values (202.77 ± 23.98 nM vs. 48.68 ± 6.45 nM), an effect that was prevented by RR (50.45 ± 13.9 nM) and was not induced by the two other CCB; (ii) NIF had a thapsigargin-like effect, because it induced the same release of intracellular calcium as TG (212.1 ± 25.62 nM); (iii) The response to NIF was reduced by 40% after the inhibition of IP3 receptor (121.21 ± 26.01 nM) and by 50% after the inhibition of tyrosine kinase (101.91 ± 7.76 nM). Together, these data demonstrate that NIF produces a deregulation of intracellular calcium homeostasis. The abnormal increase of [Ca2+]I is due to the activation of store operated channels from the plasma membrane responsible for capacitative calcium entry, a process modulated by the activity of tyrosine kinase and the Ca2+-ATPase pump from the sarcoplasmic reticulum.