Chromatin immunoprecipitation (ChIP) is a powerful method to determine whether a protein of interest binds to specific regulatory elements of the genome. Herein, we outline protocols optimized to detect binding of Hypoxia-Inducible Factor (HIF)-1α or HIF-2α to putative hypoxia response elements (HREs) within HIF target genes expressed in breast tumor epithelial cells.
CITATION STYLE
Brooks, D. L., & Seagroves, T. N. (2018). Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models. In Methods in Molecular Biology (Vol. 1742, pp. 67–79). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7665-2_7
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