Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity

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Abstract

Detergent-treatment of higher plant thylakoids with Triton X-100 at pH 6.3 has been used to purify a PS2 fraction with very high rates of oxygen evolution (1000 μmol.mg chl-1.h-1). A photosynthetic unit size of about 300 chlorophyll (chl) molecules has been determined by optical methods, suggesting an average turnover time for PS2 of about 2 ms. The donor system for P680+ is particularly well preserved in the preparation, as judged by P680+ reduction kinetics, the detection by EPR of Signal IILT and the presence of the high potential form of cytochrome b-559 (at a ratio of 1:1 with the reaction centre). © 1983.

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Ford, R. C., & Evans, M. C. W. (1983). Isolation of a photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity. FEBS Letters, 160(1–2), 159–164. https://doi.org/10.1016/0014-5793(83)80957-0

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