Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Kalweit, A., Przybilski, R., Seehafer, C., De La Peña, M., & Hammann, C. (2012). Characterization of hammerhead ribozyme reactions. Methods in Molecular Biology, 848, 5–20. https://doi.org/10.1007/978-1-61779-545-9_2
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