The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol for the heterologous expression of phytoplasma membrane proteins in Escherichia coli is described, together with a procedure to purify a suitable quantity of fusion antigen for application in the study of phytoplasma-host interactions.
CITATION STYLE
Galetto, L., Siampour, M., & Marzachì, C. (2013). Preparation of Phytoplasma Membrane Recombinant Proteins (pp. 351–369). https://doi.org/10.1007/978-1-62703-089-2_30
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