Analysis of progranulin-mediated Akt and MAPK activation

Abstract

Progranulin has emerged in recent years as an important regulator of various biological functions including cell proliferation, wound healing, motility, and protection from apoptosis. Progranulin is also critical for transformation as established in several cancer models. Progranulin biological responses elicit through the activation of the Akt and MAPK pathways, which are critical for progranulin downstream signaling. In this chapter various experimental approaches aiming at detecting progranulin-mediated Akt and MAPK activation will be discussed.