Improving the clinical significance of preclinical immunotherapy studies through incorporating tumor microenvironment-like conditions

15Citations
Citations of this article
19Readers
Mendeley users who have this article in their library.

Abstract

Frequently, the results generated when testing novel antitumor mmunotherapies in vitro do not correlate with data collected in n vivo models and/or in clinical settings. It is our hypothesis that his discrepancy is caused by the use of in vitro conditions, such as ormoxia, a two-dimensional surface, optimal growth media, and ack of cell complexity and heterogeneity. These conditions do not ccurately reflect the tumor microenvironment (TME) that the ested immunotherapeutic strategies experience in vivo. While there re many variables which can have an impact upon the antitumor fficacy of an immunotherapy, the immunosuppressive TME is one n which several of the conditions commonly found in vivo can be mimicked in vitro. These conditions, which include hypoxia, low pH, low glucose, presence of adenosine, cell complexity and heterogeneity, as well as the three-dimensional structure of TME, can all affect immune cell-tumor cell interactions. Here, we discuss the impact that these conditions, either individually or in combination, can have on these interactions. Furthermore, we propose that performing in vitro assays under TME-like conditions improves the clinical relevance of the yielded results. This, in turn, contributes to accelerate the speed, reduce the cost, and increase efficiency of screening novel immunotherapies and eventually the development of prospective clinical trials.

References Powered by Scopus

The Warburg Effect: How Does it Benefit Cancer Cells?

3393Citations
N/AReaders
Get full text

The extracellular matrix at a glance

3159Citations
N/AReaders
Get full text

The biology and function of fibroblasts in cancer

3045Citations
N/AReaders
Get full text

Cited by Powered by Scopus

CAR T Cell-Based Immunotherapy for the Treatment of Glioblastoma

102Citations
N/AReaders
Get full text

HLA class I antigen processing machinery defects in antitumor immunity and immunotherapy

41Citations
N/AReaders
Get full text

Employing hypoxia characterization to predict tumour immune microenvironment, treatment sensitivity and prognosis in hepatocellular carcinoma

24Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Maggs, L., & Ferrone, S. (2020). Improving the clinical significance of preclinical immunotherapy studies through incorporating tumor microenvironment-like conditions. Clinical Cancer Research, 26(17), 4448–4453. https://doi.org/10.1158/1078-0432.CCR-20-0358

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

100%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 5

63%

Chemistry 1

13%

Engineering 1

13%

Agricultural and Biological Sciences 1

13%

Save time finding and organizing research with Mendeley

Sign up for free