In vitro cytotoxicity of the bile acids and bile acid derivatives

Abstract

Polymeric dental resin materials are known to leach cytotoxic unreacted monomers and degradation products. In this study, methacrylic derivatives of bile acids have been synthesized for use as monomers in dental composites, their in vitro cytotoxicity and bile acids toward NIH3T3 fibroblasts has been evaluated by colorimetric MTT assay and compared with that of the common dental monomers BisGMA and TEGDMA. The results show that bile acids and their derivatives can induce mitochondrial dysfunction at similar or higher concentrations than the commercial dental monomers. Certain monomers did not influence colorimetric absorbance in MTT assay against the normal control over their entire range of solubility. © Springer-Verlag Berlin Heidelberg 2014.