Structure-based search and in vitro analysis of self-cleaving ribozymes

Abstract

Detecting functional RNAs is increasingly accomplished through structure-based searches for patterns of conserved secondary structure. With large amounts of new sequencing data becoming available, there is a greater demand for efficient methods of identifying new RNAs. Here we present a method of identifying self-cleaving ribozymes and characterizing the in vitro activity. © 2012 Springer Science+Business Media, LLC.