In silico evaluation of osmoprotectants in eucalyptus transcriptome

Abstract

The synthesis of osmoprotectants is one of the main mechanisms to dribble harmful effects caused by abiotic stresses. In the present work, osmoprotectant genes were selected and used in a search for orthologs in the FORESTs database using in silico procedures. The studied genes were here analyzed for the first time in Eucalyptus, including 51 identified orthologs from nine gene families (P5CS=3, P5CR=4, TPS1=10, TPPB=8, SAT=6, OASTL=13, CMO=1, BADH=3 and INPS1=3). In general, the most represented genes were those involved in the trehalose and cysteine synthesis, probably due to their vital importance in the maintenance of cellular homeostasis. Overall expression pattern revealed significant amounts of reads in most libraries. Dendrograms based on analysis of selected genes from other flowering plants revealed no clear separation between mono and dicots, since diversity regarding the evolution and diversity in gene signatures may be more related to fitness and adaptation, than evolutionary patterns. © 2009 Springer Berlin Heidelberg.