Bicarbonate transport along the loop of Henle I. Microperfusion studies of load and inhibitor sensitivity

71Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

We microperfused the loop of Henle (LOH) to assess its contribution to urine acidification in vivo. Under control conditions (Na HCO3- = 13 mM, perfusion rate ∼ 17 nl/min-1) net bicarbonate transport (JHCO3-) was unsaturated, flow- and concentration-dependent, and increased linearly until a bicarbonate load of 1,400 pmol·min-1 was reached. Methazolamide (2 · 10-4 M) reduced JHCO3 by 70%; the amiloride analogue ethylisopropylamiloride (EIPA) (2 · 10-4 M) reduced JHCO3 by 40%; neither methazolamide nor EIPA affected net water flux (Jv). The H+-ATPase inhibitor bafilomycin A1 (10-5 M) reduced JHCO3 by 20%; the Cl- channel inhibitor 5-nitro-2′-(3-phenylpropylamino)-benzoate (2 · 10-4 M) and the Cl--base exchange inhibitor diisothiocyanato-2,2′-stilbenedisulfonate (5 · 10-5 M), had no effect on fractional bicarbonate reabsorption. Bumetanide (10-6 M) stimulated bicarbonate transport (net and fractional JHCO3) by 20%, whereas furoscmide (10-4 M) had no effect on bicarbonate reabsorption; both diuretics reduced Jv. In summary: (a) the LOH contributes significantly to urine acidification. It normally reabsorbs an amount equivalent to 15% of filtered bicarbonate; (b) bicarbonate reabsorption is not saturated; (c) Na+-H+ exchange and an ATP-dependent proton pump are largely responsible for the bulk of LOH bicarbonate transport.

Cite

CITATION STYLE

APA

Capasso, G., Unwin, R., Agulian, S., & Giebisch, G. (1991). Bicarbonate transport along the loop of Henle I. Microperfusion studies of load and inhibitor sensitivity. Journal of Clinical Investigation, 88(2), 430–437. https://doi.org/10.1172/JCI115322

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free