Live-cell imaging

Abstract

Hypoxia Reagent. Cells were exposed to 1% O 2 levels for a period of 15 minutes prior to imaging. Under normoxic conditions (20%), there was no signal from the Image-iT Hypoxia Reagent, but in response to the decrease in oxygen levels, the signal from the Image-iT Hypoxia Reagent increased, as shown by red, punctate staining in nearly all of the cells in this image. Nuclei (blue) were visualized by using NucBlue TM Live ReadyProbes TM Reagent. Cells were imaged on the EVOS TM FL Auto Imaging System with onstage incubator using a 20x objective.