Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding

18Citations
Citations of this article
26Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Lymphatic metastasis of cancer cells involves movement from the primary tumor site to the lymph node, where the cells must be able to productively lodge and grow. It is there that tumor cells encounter cellular and non-cellular constituent elements that make up the lymph node parenchyma. Our work shows that head and neck squamous cell carcinoma (HNSCC) cell lines are able to bind to laminin, fibronectin, vitronectin, and hyaluronic acid, which are extracellular matrix elements within the lymph node parenchyma. HNSCC cell lines bound to laminin under lymphodynamic low shear stress (0.07 dynes/cm 2), consistent with lymph flow via β1 integrins, including α2β1, α3β1, and α6β1. Binding occurred in the presence of shear stress and not in the absence of flow. Additionally, tumor cell binding to laminin under flow did result in calcium signaling. Our data indicate a novel role for β1 integrin-mediated binding of HNSCC cells to laminin under conditions of lymphodynamic flow that results in intracellular calcium signaling within the cancer cell. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

References Powered by Scopus

Head and Neck Cancer

1660Citations
N/AReaders
Get full text

Structural basis of integrin regulation and signaling

1350Citations
N/AReaders
Get full text

Homing and cellular traffic in lymph nodes

1072Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Integrins as biomechanical sensors of the microenvironment

841Citations
N/AReaders
Get full text

Effect of tumor microenvironment on pathogenesis of the head and neck squamous cell carcinoma: A systematic review

330Citations
N/AReaders
Get full text

ß1 integrins as therapeutic targets to disrupt hallmarks of cancer

91Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Fennewald, S. M., Kantara, C., Sastry, S. K., & Resto, V. A. (2012). Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding. Journal of Biological Chemistry, 287(25), 21058–21066. https://doi.org/10.1074/jbc.M112.360313

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 12

63%

Researcher 5

26%

Professor / Associate Prof. 2

11%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 10

53%

Medicine and Dentistry 4

21%

Biochemistry, Genetics and Molecular Bi... 3

16%

Engineering 2

11%

Save time finding and organizing research with Mendeley

Sign up for free