Evaluation of antimicrobial activities of Garcinia Latissima Miq. Stem bark extract

Abstract

Introduction: To evaluate the antimicrobial activities of hexane, ethyl acetate, and methanol extracts from the stem bark of Garcinia latissima Miq. Method: The G. latissima Miq. was collected from Bogor. This study uses multilevel maceration extraction methods. The antimicrobial activity was determined by the good diffusion method and the broth dilution method. Result: The ethyl acetate extracts of stem bark were active against Bacillus subtilis, aeruginosa Staphylococcus aureus, and Ps aeruginosa, and virtually inactive against Escherichia coli, Candida albicans, and Trichophyton mentagrophytes. The methanol extracts of stem bark were active against B. subtilis and S. aureus, and virtually inactive against E. coli, P. aeroginosa, C. albicans, and T. mentagrophytes. The hexane extracts of stem bark were inactive against B. subtilis, S. aureus, E. coli, P. aeroginosa, C. albicans, and T. mentagrophytes. The 2% methanol extracts of G. latissima Miq. stem bark showed the maximum zone of inhibition against B. subtilis (10.70±0.638 mm), followed by the methanol extracts of G. latissima Miq. stem bark against S. aureus (10.38±0.653 mm). 2% Ethyl acetate extracts of G. latissima Miq. stem bark exhibited a maximum zone of inhibition against B. subtilis (10.35±0.867 mm) followed by P. aeruginosa (10.07±0.971 mm). The results of the antibacterial activity showed that the MeOH extract of G. latissima Miq. stem bark exhibited activities against B. subtilis (MIC/MBC = 625 ppm/2500 ppm) and S. aureus (MIC/MBC = 2500 ppm/5000 ppm). The ethyl acetate extract of G. latissima Miq. stem bark exhibited activities against P. aeruginosa (MIC/MBC = 5000 ppm/5000 ppm) and B. subtilis (MIC/MBC > 5000 ppm/5000 ppm). Conclusion: The results of the present study revealed most valuable information and also support the continued sustainable use of G. latissima Miq. stem barks in a traditional system of medicine.