LCM-seq couples laser capture microdissection of cells from frozen tissues with polyA-based RNA sequencing and is applicable to single neurons. The method utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the positional information of each cell is preserved.
CITATION STYLE
Nichterwitz, S., Benitez, J. A., Hoogstraaten, R., Deng, Q., & Hedlund, E. (2018). LCM-seq: A method for spatial transcriptomic profiling using laser capture microdissection coupled with PolyA-based RNA sequencing. In Methods in Molecular Biology (Vol. 1649, pp. 95–110). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7213-5_6
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