A One-Year Hospital Based Prospective Study of Sickle Cell Disease from One Capital Area of Kuwait by HPLC

Abstract

Background & Objectives: Haemoglobinopathies are inherited disorders of haemoglobin synthesis that are responsible for significant morbidity and mortality all over the world. Sickle cell disease (SCD) is clinically one of the most important haemoglobinopathies. The disease is characterized by red blood cell abnormality including sickling and rigidity. The aim of retrospective study was to determine the different fractions of haemoglobins that appear in a chromatogram of patients with sickle cell disease and to measure different types of haemoglobins in one capital area in 2015. Methods: HPLC records of 144 patients with SCD were retrieved from hospital records. Records from 34 control patients were also retrieved. About 178 subjects were included with mean age of 28.2 (SD 20.16) years. Results: 76.3% of patients had an HbAS phenotype, whilst 23.6% were HbSS. Irrespective of their gender or age; differences were observed in HbA, HbS and HbF levels in patients compared to controls. Interpretation & Conclusion: Knowledge of the prevalence of haemoglobin phenotype patterns underpins screening, diagnosis and treatment strategies within our population.