2,2-diphenyl-1-picrylhydrazyl (Dpph) radical scavenging activity of extracts from aloiampelos striatula

Abstract

Hexane, chloroform, acetone, methanolic and water extracts from leaves and stem-bark of Aloiampelos striatula were evaluated for their antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The hexane, chloroform, acetone, methanolic and water extracts from leaves of A. striatula showed scavenging activity ranging from 10.30±0.41-30.14±1.4, 30.49±0.85-45.95±0.22, 38.45±0.49-51.65±0.95, 34.83±1.53-64.98±0.45 and 54.37±1.24-66.74±0.89%, respectively, at various concentrations. The hexane, chloroform, acetone, methanolic and water extracts from stem-bark of A. striatula showed scavenging activity ranging from 12.53±3.07-29.81±2.43, 17.80±5.17-37.20±2.13, 6.15±0.24-62.79±3.82, 38.23±1.18-67.32±0.55 and 3.16±0.40-70.66±0.18%, respectively, at various concentrations. Additionally, the IC50 values of these extracts were also determined and were found to be in the range of <200 to >3000 µg/mL. The positive control, vitamin C, showed an IC50 value of <200 µg/mL. The therapeutic applications of A. striatula are often in traditional Basotho medicine in the Kingdom of Lesotho, which include treating skin problems, high blood pressure, sexually transmitted illnesses, common colds, blood cleansing, stomach ailments and dressing of wounds. Further studies on this plant are required to explore this plant for its commercial applications.