Experimental design, data analysis, and fluorescence quantitation

Abstract

Errors in flow cytometry can arise from two discrete processes: data acquisition and data analysis. Data acquisition errors can be avoided by good sample preparation and accurate instrument setup. Instrument standards, in the form of fluorescent beads, can be used to confirm instrument performance in both settings. The only aspect of data acquisition for which controls are unavailable is the ability of the operator to design the correct experiment. Help with this is described in this chapter.