New Application of pH-Mediated Acid Stacking Technique for Amphoteric Compounds in Capillary Electrophoresis: Example Assay of Blood Glutathiones

8Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

Both reduced and oxidized forms of glutathiones were firstly stacked and detected using pH-mediated acid stacking method, in which glutathiones were stacked as cations and separated as anions. Factors, such as injection time, sweeping time, buffer pH, concentration of sodium chloride in sample matrix, that influenced stacking and separation were systematically studied and optimized. Under the optimum condition, the enhancement factors of ~20 times for both reduced and oxidized forms of glutathiones could be easily obtained within 20 min with satisfied sensitivities (limit of detections were 0.12 and 0.06 μmol/L for reduced and oxidized glutathione, respectively, at signal-to-noise ratio, S/N = 3), linearity range (0.3-300.0 and 0.6-300.0 μmol/L for reduced and oxidized glutathione, respectively), recoveries (>98%) and reproducibilities (relative standard deviation <5.1% for peak height). The proposed method provides an alternation way for assaying of glutathiones, as well as amphoteric compounds, in blood sample.

Cite

CITATION STYLE

APA

Kong, Y., Yang, G., Kong, L., Yang, G., Li, H., Ji, X., & Gao, M. (2017). New Application of pH-Mediated Acid Stacking Technique for Amphoteric Compounds in Capillary Electrophoresis: Example Assay of Blood Glutathiones. Journal of Chromatographic Science, 55(4), 477–483. https://doi.org/10.1093/chromsci/bmw205

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free