Understanding the complex genetic background of cancers is key in developing effective targeted therapies. The Sleeping Beauty (SB) transposon system is a powerful and unbiased genetic editing tool that can be used for rapid screening of candidate liver cancer driver genes. Manipulating their expression level using a reverse genetic mouse model involving hydrodynamic tail-vein injection delivery can rapidly elucidate the role of these candidate genes in liver cancer tumorigenesis.
CITATION STYLE
Chiu, A. P., & Keng, V. W. (2019). Liver-Specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation. In Methods in Molecular Biology (Vol. 1907, pp. 185–196). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8967-6_15
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