Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the fetal-maternal interface and the peripheral blood

Abstract

The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and pre-eclampsia. We found total T cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the fetal-maternal interface. Defining the functional nature and specificity of compartment-specific T cells will be necessary if we are to understand localized immunity, tolerance, and pathogenesis. © 2014 Neller, Santner-Nanan, Brennan, Hsu, Joung, Nanan, Burrows and Miles.