In the last two decades, phage display technology has been used for investigating complex biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical cloning and expression system, has also been exploited for generating display libraries of small peptides and protein domains. More recently, large cDNA and whole-genome lambda display libraries of human pathogens have been generated for the discovery of new antigens for biomedical applications. Here, we describe the construction of a whole-genome library of a common pathogen—Streptococcus pneumoniae—and the use of this library for the molecular dissection of the human B-cell response against bacterial infection and colonization.
CITATION STYLE
Beghetto, E., & Gargano, N. (2019). Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery. In Methods in Molecular Biology (Vol. 2024, pp. 181–198). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9597-4_11
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