In vitro shoot proliferation and plant regeneration of phlogacanthus thyrsiflorus nees. a rare medicinal shrub of Bangladesh

Abstract

An efficient protocol was developed for shoot proliferation and plant regeneration ofPhlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub ofBangladesh, through in vitro culture using shoot tip and nodal explants. Best shootinduction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2%of nodal explants responded to produce maximum number (12.4 ± 0.66) of shootsper culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA +0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rootingculture tubes were kept in normal room temperature for 7 days beforetransplanting in pots where plantlets were reared for three weeks. The survivalrate of regenerated plantlets was 85%.