The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.
CITATION STYLE
Prasannan, P., & Verma, N. K. (2019). Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis. In Methods in Molecular Biology (Vol. 1930, pp. 51–57). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9036-8_7
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