The use of menadione as an intermediate hydrogen-carrier for measuring cytoplasmic dehydrogenating enzyme activities

Abstract

Phenazine methosulphate is often used as an intermediate hydrogen-carrier in the demonstration of dehydrogenase activities. It cannot be used with adrenal tissue sections because it appears to have an inhibitory effect in this tissue. The water-soluble menadione sodium-bisulphite salt has been shown to be quantitatively as efficient as phenazine methosulphate for pentose-shunt dehydrogenase activity in rat liver sections; at optimal concentrations it does not inhibit this activity in the adrenal. © 1973 Springer-Verlag.