Five current methods of metallothionein (MT)-estimation in biological materials (Hg/TCA-, Cd/heme-, SH-assay, G 75-method and RIA) were compared. Uniform calibration was ensured by measuring the nitrogen-content of the standard-apoprotein after "Kjeldahl-digestion". Known amounts of rabbit standard Cd-MT I were recovered from identical rat S9-samples. In an overall rating, RIA and Cd/heme-assay performed best, whereas the other methods either underestimated (SH-, G 75) or overestimated (Hg/TCA) the 100%-expectation.
CITATION STYLE
Dieter, H. H., Müller, L., Abel, J., & Summer, K. H. (1987). Metallothionein-determination in biological materials: interlaboratory comparison of 5 current methods. Experientia. Supplementum, 52, 351–358. https://doi.org/10.1007/978-3-0348-6784-9_33
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