DNA transfection mediated by cationic lipid reagents

Abstract

Liposomal transfection reagents vary in their ability to transfect cell lines efficiently. Some are gener-alists, whereas others are best used with specific cell types. The nonliposomal FuGENE 6 and the cationic liposomal Lipofectamine 2000 are examples of reagents that can successfully transfect most adherent and suspension cell types (including several primary and hard-to-transfect cell types) with negligible toxicity and a minimal number of manipulations. Importantly, both reagents can be used to transfect cells in the presence of serum, minimizing the number of manipulations during the transfection procedure. We also provide an alternative protocol that uses the cationic lipid reagents Lipofectin and Transfectam.