Evaluation of the Antioxidant and Anti-diabetic Effect of Mucuna puriens Extract

  • Njemuwa N
  • Dickson N
  • Elizabeth A
  • et al.
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Abstract

Aims: To investigate the scientific basis for the anti-diabetic and antioxidant activity of Mucuna pruriens (Fabaceae) leaf ethanolic extract using alloxan-induced diabetic rats, DPPH and ABTS assay. Place and Duration of Study: Department of Chemistry/Biochemistry Federal University Ndufu Alike, Ebonyi State, Nigeria between October 2013 and May 2014. Methodology: The polyphenol content was determined using Folin-Ciocaltu method and their linear relationship with antioxidant activity was evaluated using linear regression analysis. The antioxidant activity was determined using 1, 1-Diphenyl, 2-picrylhydrazyl (DPPH) and (2, 2-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical assay. The active ingredients in the ethanolic extract were isolated using HPLC method. Also the ant-diabetic activity was determined in vivo using alloxan-induced diabetic wister rats.   Results: Ethanol extract showed the highest phenolic content as well as highest antioxidant activity. A strong relationship was found between phenolic contents and antioxidant activity. The HPLC analysis indicates the presence of gallic acid, caffeic acid, p-coumaric acid, quercetin and (+)-catechin. The ethanolic extract at the concentration of 400 mg/kg significantly (P<0.001) increased the intracellular antioxidant enzymes and reduced the elavated serum lipids and showed more active than the reference drug (metformin). Conclusion: Based on the obtained result, the antioxidant and anti-diabetic activity demonstrated by Mucuna pruriens leaf extracts provide good evidence to support the traditional use of this plant in treatment of diabetics.

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Njemuwa, N. N., Dickson, N. U., Elizabeth, A. E., Uchenna, R. M., & Ogbonnaya, C. N. (2019). Evaluation of the Antioxidant and Anti-diabetic Effect of Mucuna puriens Extract. European Journal of Medicinal Plants, 1–9. https://doi.org/10.9734/ejmp/2019/v27i230110

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