Isolation of glucosamine hcl from scylla paramamosain and determination by HPLC

Abstract

Scylla paramamosain is one of crustaceans that has economic value in Indonesia. About 40-50% of the total weight of crustaceans goes as waste after being processed to be human food. The Crustacean waste needs to be reduced in order to minimize the negative impact on the environment. Whereas products derived from crustacean shell also has medical value (e.g. Chitosan, Glucosamine HCl). Glucosamine is an amino monosaccharide acting as a substrate for the production of aggrecan and proteoglycans and thus have therapeutic activity in osteoarthritis. The present study has been aimed to prepare glucosamine hydrochloride (GluHCl) from S. paramamosain waste by acid hydrolysis in four treatment groups and their quantitation by high-performance liquid chromatography (HPLC). The best condition from this experiment is shell deproteination with 3% NaOH solution (w/v 1:6), at 85ºC, for 30 minutes, demineralisation with 1N HCl (w/v 1:10), at 75ºC, for 1.5 hours, depigmentation with NaClO 0.38%, at room temperature, for 1 hour, and hydrolysis with conc. HCl (w/v 1:20), at 85ºC, for 18 minutes, resulted in the yield of 6.15 ± 0.62% and quantitation by HPLC analysis of obtained glucosamine hydrochloride was 98.48 ± 0.74%. Further, this study describes the recycling of S. paramamosain waste to a value-added product which is having potential applications in the field of food and medicine.