The monocytes (MONOs) can be considered as “double-edge swords”; they have both important pro- inflammatory and anti-inflammatory functions manifested in part by cytokine production and release. Although MONOs are circulating cells, they are the major precursors of a variety of tissue-specific immune cells such as the alveolar macrophage, dendritic cells, microglial cells, and Kupffer cells. Unlike the polymorphonuclear leukocyte, which produces no or very little interleukin-10 (IL-10), the monocyte can produce this potent anti-inflammatory cytokine to control inflammation. IL-10, on an equimolar basis, is a more potent inhibitor of pro-inflammatory cytokines produced by monocytes than many antiinflammatory glucocorticoids which are used clinically. This chapter describes how to isolate monocytes from human blood and the use of IL-10 monoclonal antibody to determine the effect and timing of endogenous IL-10 release on the production and release of pro-inflammatory cytokines.
CITATION STYLE
Patel, H., & Davidson, D. (2014). Control of pro-inflammatory cytokine release from human monocytes with the use of an interleukin-10 monoclonal antibody. Methods in Molecular Biology, 1172, 99–106. https://doi.org/10.1007/978-1-4939-0928-5_8
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