Control of Black Spot Disease by Ultraviolet-B Irradiation in Rose (Rosa × hybrida) Production

Abstract

We investigated the effect of ultraviolet-B (UV-B) irradiation on the development of black spot disease caused by Diplocarpon rosae Wolf., which is a major problematic disease in rose (Rosa × hybrida) production. The growth of D. rosae colonies was suppressed on potato dextrose agar (PDA) medium under UV-B irradiation (peak wavelength: 310 nm; full width at half maximum: 30 nm) at an intensity of 15 μW·cm−2 with 1 h daily treatment. In addition, black spot conidia were inoculated to the rose ‘Danjiri Bayashi’ leaves and the effective growth suppression of black spot symptoms was observed on the leaves under UV-B irradiation. Next, various rose cultivars were planted in two greenhouses: one for supplemental UV-B irradiation treatment and one as a control without the treatment. In the UV-B irradiation greenhouse, the roses were irradiated at an intensity of 3–5 μW·cm−2 every day from 23:00–23:30 and 0:00–0:30 (total: 1 h). No chemical pesticides other than a starch agent for aphid control were used throughout the experiment. With the exception of the data for ‘Papa Meilland’ in 2019, UV-B irradiation significantly reduced the number of leaves infected with black spot disease. In September 2019, the non-UV-B irradiated ‘Danjiri Bayashi’ and ‘Papa Meilland’ had severe black spot symptoms on over 20 leaves. The number of plants with black spot symptoms increased in July 2020 compared to 2019. On the other hand, in UV-B irradiated plants, fewer black spot symptoms were observed than in non-UV-B irradiated plants. Although some visible damage was observed in the UV-B irradiated plants, the chlorophyll and carotenoid contents in the leaves decreased, indicating that UV-B irradiation had a certain negative effect on the photosynthetic apparatus. Over a five-month period, the cumulative number of flowers in the UV-B irradiation greenhouse did not decrease, and actually increased, depending on the cultivar, compared to the control treatments. Our results suggest that supplemental UV-B irradiation is effective at suppressing black spot disease in roses and can contribute to the production of pesticide-free edible rose production.