Evolution of genome size and chromosome number in the carnivorous plant genus Genlisea (Lentibulariaceae), with a new estimate of the minimum genome size in angiosperms

Abstract

• Background and Aims Some species of Genlisea possess ultrasmall nuclear genomes, the smallest known among angiosperms, and some have been found to have chromosomes of diminutive size, which may explain why chromosome numbers and karyotypes are not known for the majority of species of the genus. However, other members of the genus do not possess ultrasmall genomes, nor do most taxa studied in related genera of the family or order. This study therefore examined the evolution of genome sizes and chromosome numbers in Genlisea in a phylogenetic context. The correlations of genome size with chromosome number and size, with the phylogeny of the group and with growth forms and habitats were also examined. • Methods Nuclear genome sizes were measured from cultivated plant material for a comprehensive sampling of taxa, including nearly half of all species of Genlisea and representing all major lineages. Flow cytometric measurements were conducted in parallel in two laboratories in order to compare the consistency of different methods and controls. Chromosome counts were performed for the majority of taxa, comparing different staining techniques for the ultrasmall chromosomes. • Key Results Genome sizes of 15 taxa of Genlisea are presented and interpreted in a phylogenetic context. A high degree of congruencewas found between genome size distribution and the major phylogenetic lineages. Ultrasmall genomes with 1C values of ,100 Mbp were almost exclusively found in a derived lineage of South American species. The ancestral haploid chromosome number was inferred to be n = 8. Chromosome numbers in Genlisea ranged from 2n = 2x = 16 to 2n = 4x = 32. Ascendant dysploid series (2n = 36, 38) are documented for three derived taxa. The different ploidy levels corresponded to the two subgenera, butwere not directly correlated to differences in genome size; the three different karyotype ranges mirrored the different sections of the genus. The smallest knownplant genomeswere not found in G. margaretae, as previously reported, but in G. tuberosa (1C≈61 Mbp)and some strains of G. aurea (1C ≈ 64 Mbp). •Conclusions Genlisea is an ideal candidate model organism for the understanding ofgenomereduction as the genus includes species with both relatively large (~1700 Mbp) and ultrasmall (~61 Mbp) genomes. This comparative, phylogeny-based analysis ofgenomesizes and karyotypes in Genlisea provides essential data for selection of suitable species for comparative whole-genome analyses, aswell as for further studies on both the molecular and cytogenetic basis of genome reduction in plants.