Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.
CITATION STYLE
Teal, E., Bertaux-Skeirik, N., Chakrabarti, J., Holokai, L., & Zavros, Y. (2018). Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids. In Methods in Molecular Biology (Vol. 1817, pp. 145–155). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8600-2_15
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