Microfluidic blood cuvette for hemoglobin measurement

Abstract

This paper presents hemoglobin measurement based on microfluidic blood cuvettes. Blood sample filled MBC by capillary action, and hemoglobin in the blood sample was measured by absorbance of 530nm-wavelenth light. Microfluidic blood cuvette was fabricated by poly-methylmethacrylate (PMMA) substrate and dry film photoresist (DFR). Two different MBC including dual-side wall and sing-side wall microchannels were designed and fabricated. During blood filling process, air was trapped in microfluidic blood cuvette of dual-side wall microchannel, while there was no air trapping in microfluidic blood cuvettes of single-side wall microchannel. Sensitivity of hemoglobin measurement was more linear in 105(μm-depth microchannel rather than 35(μm-depth microchannel. © 2013 IFMBE.