Abstract
A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.
Cite
CITATION STYLE
Birikh, K. R., Bernad, P. L., Shmanai, V. V., Malakhov, A. D., Shchepinov, M. S., & Korshun, V. A. (2009). SNP detection using trityl mass tags. Methods in Molecular Biology (Clifton, N.J.), 578, 345–361. https://doi.org/10.1007/978-1-60327-411-1_21
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
