Purifying progranulin may be useful in a variety of situations, for example, after it has been mutated or otherwise modified or when working with a species for which commercially produced progranulin is unavailable. A method to express and purify human progranulin is presented. Progranulin is transiently expressed in mammalian cells and isolated from their conditioned medium before purification by reversed-phase high-performance liquid chromatography (RP-HPLC). Human progranulin is used as an example, but the protocol can be applied to any other progranulin protein. Modifications of the expression-purification strategy for metabolic labeling of progranulin and analytical systems based on heparin-affinity chromatography are presented.
CITATION STYLE
Zhao, C. Y., & Bateman, A. (2018). Methods for expression and purification of biologically active recombinant progranulin. In Methods in Molecular Biology (Vol. 1806, pp. 35–49). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8559-3_3
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