Effect of Different Inoculation Methods and Inoculum Levels of Macrophomina phaseolina on Okra

Abstract

Among two methods of Macrophomina phaseolina inoculation used for pathogenicity test, soil infestation method comparatively checked more plant growth of okra plants than seed infestation method. Minimum plant length and weight, as well as seed germination were observed by soil infestation method. Significantly maximum plant mortality and root infection was also occurred in soil infestation method. Seed germination, plant growth, plant mortality and root infection of okra plants were adversely affected with the increasing inoculum levels of M. phaseolina. Seed germination and plant growth were negatively correlated with inoculated pathogen population; whereas, plant mortality and root infection were positively correlated with the inoculum level of M. phaseolina.