Protists are mostly unicellular eukaryotes. Some protists are beneficial for plants, while others live as endosymbionts and can cause severe plant diseases. More detailed studies on plant-protist interactions exist only for plant pathogens and parasites. A number of protists live as inconspicuous endophytes and cause no visible disease symptoms, while others appear closely associated with the rhizosphere or phyllosphere of plants, but we still have only a vague understanding on their identities and functions. Here, we provide a protocol on how to assess the plant-associated protist community via Illumina-sequencing of ribosomal marker-amplicons and describe how to assign taxonomic affiliation to the obtained sequences.
CITATION STYLE
Dumack, K., & Bonkowski, M. (2021). Protists in the plant microbiome: an untapped field of research. In Methods in Molecular Biology (Vol. 2232, pp. 77–84). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1040-4_8
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