The purpose of this study is to elucidate the potential role of interleukin (IL)-10+ regulatory B cells and other B cell subsets in the development of hepatitis B virus-associated membranous nephropathy (HBV-MN). A total of 14 patients with new onset HBV-MN, 12 individuals with immune-tolerant HBV infection (HBV-IT), and 12 healthy controls (HC) were examined for the percentages of CD38+, CD86+, CD27+, CD95+ and IL-10+ B cells by flow cytometry. Serum IL-10 concentration was examined by enzyme-linked immunosorbent assay (ELISA). The percentages of CD38+CD19+, CD86+CD19+, CD38+CD86+CD19+, and CD95+CD19+ B cells were significantly higher in HBV-MN patients than the HBV-IT and HC. The percentages of CD5+CD19+, IL-10+CD19+ B cells and serum IL-10 level in HBV-MN patients were significantly higher than the HC, and lower than the HBV-IT. Percentages of CD38+CD19+, and CD86+CD19+ B cells were reduced after treatment, while the percentages of CD5+CD1d+CD19+, CD5+CD1d+IL-10+CD19+, and IL-10+CD19+ B cells were increased. The 24 h urinary protein concentration was positively correlated with the percentage of CD38+CD19+, and negatively correlated with the percentage of IL-10+CD19+ B cells and serum IL-10 level. Similarly, the value of eGFR was negatively correlated with the percentage of CD38+CD19+, and positively correlated with the percentage of IL-10+CD19+ B cells and serum IL-10 level. Serum IL-10 level and the percentage of IL-10+CD19+ were negatively correlated with the percentages of CD38+CD19+, and CD86+CD19+ B cells. These results suggest that CD86+CD19+, CD38+CD86+CD19+, CD95+CD19+, and especially CD38+CD19+ and IL-10+CD19+ cells may participate in the pathogenesis of HBV-MN.
CITATION STYLE
Liu, Y., Wang, H., Hu, X., Qu, Z., Zhang, H., Crew, R., … Jiang, Y. (2016). A higher frequency of IL-10-producing B cells in Hepatitis B virus-associated membranous nephropathy. Clinical and Experimental Pharmacology and Physiology, 43(4), 417–427. https://doi.org/10.1111/1440-1681.12552
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