Nitrogen use aggravates bacterial diversity and network complexity responses to temperature

Abstract

Rising temperature affects microbial composition and function in agriculture field, especially under nitrogen fertilization. In this study, we investigated the bacterial community of paddy soil incubated at controlled temperatures (5 °C, 15 °C, 25 °C, and 35 °C). Results showed that the response of bacterial communities to temperature was not uniform. Temperature elevation from 15 to 25 °C abruptly shifted the soil bacterial community, whereas elevation from 5 to 15 °C and from 25 to 35 °C had a marginal effect. The bacterial α-diversity was higher at 5 °C and 15 °C, owing to the massively distributed taxa with low abundance. However, as the temperature increased to 25 °C and 35 °C, these taxa were diminished, whereas Firmicutes significantly increased, resulting in a strong decline in α-diversity. Simultaneously, bacterial network complexity significantly increased at 25 °C and 35 °C, indicating the bacteria had closer interactions. Nitrogen application aggravated the variation in bacterial diversity and network complexity among temperatures. Interestingly, most complex network was observed under higher temperatures in fertilized soils. Collectively, these results indicate that nitrogen exacerbates the response of the soil bacterial community to temperature, and association between diversity and network complexity may be present.