High-pressure microscopy is a powerful technique for visualizing the effects of hydrostatic pressure on research targets. It can be used for monitoring the pressure-induced changes in the structure and function of molecular machines in vitro and in vivo. This chapter focuses on the use of high-pressure microscopy to measure the dynamic properties of molecular machines. We describe a high-pressure microscope that is optimized both for the best image formation and for stability under high hydrostatic pressure. The developed system allows us to visualize the motility of ATP-driven molecular motors under high pressure. The techniques described could be extended to study the detailed mechanism by which molecular machines work efficiently in collaboration with water molecules.
CITATION STYLE
Nishiyama, M. (2018). Controlling the motility of ATP-driven molecular motors using high hydrostatic pressure. In The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery (pp. 325–337). Springer Singapore. https://doi.org/10.1007/978-981-10-8459-1_19
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