Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunity to develop novel personalized treatment options for numerous diseases and to advance current approaches for cell-based drug discoveries and disease modeling. The ability to differentiate iPSCs into relevant cell types is an important prerequisite for the successful development of iPSC-based treatment and modeling strategies. Here, we describe a protocol for the efficient differentiation of human iPSCs into functional keratinocytes. The protocol employs treating iPSCs with retinoic acid and bone-morphogenetic protein-4 to induce differentiation toward a keratinocyte lineage, which is then followed by the growth of differentiated iPSCs on collagen type I- and collagen type IV-coated dishes to enrich for iPSC-derived keratinocytes.
CITATION STYLE
Kogut, I., Roop, D. R., & Bilousova, G. (2013). Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage (pp. 1–12). https://doi.org/10.1007/7651_2013_64
Mendeley helps you to discover research relevant for your work.