Estimation of catechin in Ayurvedic oil formulations containing Acacia catechu

Abstract

A sensitive, simple, rapid, and efficient HPTLC method was developed and validated for the analysis of catechin in marketed Ayurvedic oil formulations containing Acacia catechu. Chromatography of methanolic-0.1% formic acid (7:3, v/v) extracts of these formulations was performed on silica gel 60 F 254 aluminum-backed TLC plates of 0.2 mm layer thickness. The plate was developed up to 85 mm with the ternarymobile phase chloroform-acetone-0.1% formic acid (7.7 + 1.5 + 0.8%, v/v/v) at 22 ± 2°C with 20 min of chamber saturation. The system produced compact spots of catechin at an R f value of 0.36. The marker, catechin, was quantified at its maximum absorbance of 296 nm. The limit of detection and quantitation values were 6 and 20 ng/spot, respectively. The linear regression analysis data for the calibration plot showed a good linear relationship with a correlation coefficient of 0.9993 in the concentration range of 200-1200 ng/spot for catechin with respect to peak area. Repeatability of the method was 0.88% RSD. Recovery values from 97 to 102% indicate excellent accuracy of the method. The developed HPTLC method is accurate, precise, and cost-effective, and it can be successfully applied for the determination of catechin in marketed Ayurvedic oil formulations containing Acacia catechu.