Infrared (IR) spectroscopy is a widely used technique for evaluation of protein secondary structure. In this chapter, we focus on the application of this analytical technique for analysis of inclusion bodies. After a general introduction to protein analysis by IR spectroscopy, different approaches for spectra acquisition, data processing, and secondary structure evaluation are presented.
CITATION STYLE
Schwaighofer, A., & Lendl, B. (2023). Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies. Methods in Molecular Biology (Clifton, N.J.), 2617, 209–223. https://doi.org/10.1007/978-1-0716-2930-7_15
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