Detection, segmentation, and quantification of individual cell nuclei is a standard task in biomedical applications. Due to the increasing volume of acquired image data, it is not possible to rely on manual labeling and object counting. Instead, automated image processing methods have to be applied. Especially in three-dimensional data, one of the major challenges is the separation of touching cell nuclei in densely packed clusters. In this paper, we propose a method for automated detection and segmentation of immunostained cell nuclei in ultramicroscopy images. Our algorithm utilizes interactive learning and voxel classification to obtain a foreground segmentation and subsequently performs the splitting process for each cluster using a multi-step watershed approach. We have evaluated our results using reference images manually labeled by domain experts and compare our approach to state-of-the art methods.
CITATION STYLE
Scherzinger, A., Kleene, F., Dierkes, C., Kiefer, F., Hinrichs, K. H., & Jiang, X. (2016). Automated segmentation of immunostained cell nuclei in 3D ultramicroscopy images. In Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) (Vol. 9796 LNCS, pp. 105–116). Springer Verlag. https://doi.org/10.1007/978-3-319-45886-1_9
Mendeley helps you to discover research relevant for your work.