Sanger Sequencing and Next-Generation Gene Sequencing: Basic Principles and Applications in Pathology

Abstract

DNA sequencing indicates the determination of the order of sequence of the base pair in DNA. Sanger sequencing is a widely used commercial DNA sequencing. The chapter elucidates the basic principle and steps of Sanger sequencing. Next-generation sequencing (NGS) is the newer gene sequencing technology that rapidly performs sequencing from abundant small fragments of DNA. The different types of NGS include pyrosequencing, microelectrophoretic methods, hybridization sequencing and real-time observation of single molecules. The basic principles, advantages and limitation of these techniques have been discussed in this chapter.