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Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

MicrobiologyOpen (2019) 8(8)
DOI: 10.1002/mbo3.797
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Abstract

Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3O4-SiO2 iron beads and was confirmed via SDS-PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6-HSL. This study opens a new avenue to actively immobilize enzymes via magnetic interaction and quench quorum sensing.

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APA

Wang, L., Xu, H., Liu, Z., Sun, T., Yuan, C., Yang, Y., … Xie, H. (2019). Magnetic immobilization of a quorum sensing signal hydrolase, AiiA. MicrobiologyOpen, 8(8). https://doi.org/10.1002/mbo3.797

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