Diversity and Origin of Indigenous Village Chickens ( Gallus gallus) from Chad, Central Africa

Abstract

In this study we assess the maternal genetic diversity and origin of indigenous village chickens from Chad complementing previous phenotypic and biometric measurements studies. We analysed a 387 bp fragment of the mitochondrial DNA (mtDNA) D-loop region of 181 village chickens from three populations of western Chad (Lake Chad/Hadjer Lamis), central Chad (Guera) and south-west Chad (Pala) and at different poultry markets in N'Djamena. Twenty-five polymorphic sites and 20 haplotypes are identified. Phylogenetic and network analyses group all chicken into a single mtDNA haplogroup D. Comparison with reference sequences shows that this haplogroup is the commonest one observed in chicken and it supports the Indian subcontinent as the maternal center of origin for the village chicken in Chad. Little genetic variation was found within and between populations which is in agreement with a recent and a maternal founding effect for the chicken in the country.